Polypeptides are bioactive substances related to various cellular functions in organisms and are compounds formed by multiple amino acids combined through peptide bonds in a certain sequence.

In general, polypeptide synthesis is divided into two types: liquid-phase polypeptide synthesis and solid-phase synthesis. Liquid-phase synthesis, as the name implies, is polypeptide synthesis carried out in a solution. Solid-phase synthesis refers to polypeptide synthesis on a solid carrier. Nutsche filter reactor can perfectly meet the conditions required for the reaction in terms of structural design. So, what are the advantages of using Nutsche filter reactor for polypeptide synthesis?

1. Stirring and Mixing

When using the Fmoc protecting group strategy, amino acids with Fmoc protecting groups and solid-phase resin can be placed in the reaction kettle. The design of the stirring paddle enables the resin to be uniformly dispersed and fully swollen in the solvent, making it easier for amino acids to react with the active groups on the resin to form stable covalent bonds.

2. Precise Temperature Control

When piperidine removes the Fmoc protecting group, the jacket design of the reaction kettle can allow the passage of heat transfer oil to maintain the required temperature, which is usually carried out at room temperature or a slightly higher temperature. At the same time, through a reasonable stirring speed, the deprotection reaction proceeds quickly and uniformly, ensuring that the Fmoc protecting groups on each amino acid can be effectively removed, exposing the amino groups for the next coupling reaction.

3. Filtration and Washing

 After each step of amino acid coupling and protecting group removal, the resin needs to be washed with an appropriate solvent. The filtration device equipped in the solid-phase synthesis reaction kettle, such as a sand core filter plate, can easily achieve solid-liquid separation, filter out the solvent containing impurities, and then add new solvents for multiple washings until the impurities on the resin are completely removed.

Overall, Nutsche filter reactor can meet diverse synthesis needs and are suitable for the solid-phase synthesis of various conventional linear polypeptides and polypeptides with special structures or functions. They can synthesize polypeptides of different sequences and structures to meet the needs of different fields such as drug research and development and biomedical research.